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KMID : 0644120180310040001
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Journal of Oriental Gynecology
2018 Volume.31 No. 4 p.1 ~ p.15
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Anti-osteoporotic Activity of Mixed Herbal Extract Involving Platycodon Grandiflorum Root in Osteoblastic MC3T3-E1 and Osteoclastic RAW 264.7 cells
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Jung Jae-In
Lee Hyun-Sook
Kim Hyung-Jun
Kim Yong-Min
Kim Soo-Hyun
Yoo Dong-Jin
Kim Eun-Ji
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Abstract
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Objectives: Osteoporosis is considered a serious human disease. We developed an extract of mixed herbs containing root of Platycodon grandiflorum (ExMH-PGR), which is expected to be effective in preventing or treating osteoporosis. The aim of this study was to investigate the anti-osteoporotic effect of ExMH-PGR in osteoblastic MC3T3-E1 cells and osteoclastic RAW 264.7 cells.
Methods: To examine the anti-osteoporotic effect of ExMH-PGR, osteoblast and osteoclast differentiation were induced and cultured with various concentrations of ExMH-PGR. Alkaline phosphatase (ALP) activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells were analyzed. Tartrate-resistant acid phosphatase (TRAP) activity and the formation of actin ring in RAW 264.7 cells were analyzed.
Results: ExMH-PGR at concentration up to 25 μg/mL significantly increased ALP activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells. ExMH-PGR at 50 to 200 μg/mL significantly inhibited TRAP activity and the formation of actin ring in RAW 264.7 cells.
Conclusions: These results demonstrate that ExMH-PGR stimulates osteoblastic activities and inhibits osteoclastic activities in in vitro systems, suggesting that ExMH-PGR might be considered as an anti-osteoporotic candidate for treatment of osteoporosis disease.
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KEYWORD
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Osteoporosis, Osteoblast, Osteoclast, Medicinal Herb, Platycodon Grandiflorum
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